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A-LevelBiologyInherited change9700/42 · May/June 2018 · Q210 Marks

In the sweet pea plant, Lathyrus odoratus, one gene codes for flower colour and one gene codes for pollen grain shape. Flower colour is either purple or red. Pollen grain shape is either long or round. The inheritance of these genes is an example of autosomal linkage. • The allele F for purple flowers is dominant over the allele f for red flowers. • The allele G for long pollen grains is dominant over allele g for round pollen grains.

  1. (a)Explain the meaning of the term autosomal linkage.[2]
  2. (b)(i)A dihybrid cross was carried out between homozygous dominant and homozygous recessive sweet pea plant parents to produce the F1 generation. The offspring from the F1 generation were crossed to produce the F2 generation. Draw a genetic diagram to show a dihybrid cross between two offspring from the F1 generation. Assume that these genes are closely linked and that there are no crossing over events.[4]
  3. (b)(ii)The actual results of the dihybrid cross are shown in Table 2.1. Table 2.1 phenotypes of F2 offspring number of individuals purple flowers, long pollen grains 284 purple flowers, round pollen grains 21 red flowers, long pollen grains 21 red flowers, round pollen grains 55 State how the results support the fact that this is an example of autosomal linkage.[1]
  4. (c)(i)In a test cross, an individual of known genotype is crossed with an individual that has a dominant phenotype but unknown genotype. State the genotype of the known individual in a test cross.[1]
  5. (c)(ii)A test cross was carried out with sweet pea plants known to be heterozygous for both flower colour and pollen grain shape. The results of the test cross are shown in Table 2.2. Table 2.2 phenotypes of offspring of test cross number of individuals purple flowers, long pollen grains 215 purple flowers, round pollen grains 30 red flowers, long pollen grains 32 red flowers, round pollen grains 210 The result of a test cross can be used to determine a crossover value (COV). A crossover value is the percentage of the total number of offspring showing recombination. The crossover value (COV) can be calculated using the formula shown in Fig. 2.1. COV = number of recombinants / total number of individuals × 100 Fig. 2.1 Calculate the COV from the results shown in Table 2.2. COV = ....................................................%[1]
  6. (c)(iii)Suggest what information about the relative distance between the linked genes can be gained from crossover values.[1]

Mark scheme

Marking points from the official mark scheme (May/June 2018). The mark or mark type is shown in brackets.

  1. [2] (autosomal) not a sex chromosome ; (linkage) genes on the same chromosome / alleles inherited together;
  2. [4] 1 F1 genotypes FG/fg or FGfg or (FG) (fg); A FfGg 2 gametes FG fg FG fg; 3 F2 genotypes FGFG FGfg FGfg fgfg; A FFGG FfGg FfGg ffgg 4 F2 phenotypes purple purple purple red long long long round ecf for mp3 from incorrect gametes (mp2) R if sex linkage
  3. [1] any 1 of: do not show 9:3:3:1 ratio ; large(r) numbers of parental phenotypes / low(er) numbers of recombinant phenotypes ;
  4. [1] fgfg / homozygous recessive ; A ffgg
  5. [1] 12.7 or 13 ;
  6. [1] low, COV / crossover value, indicates genes closer together or high, COV / crossover value, indicates genes further apart ;

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About this question

Topic
Inherited change, A-Level Biology (syllabus 9700). Structured question, 10 marks.
Source
A-Level Biology, May/June 2018 Paper 4 Variant 2, question 2.

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