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A-LevelBiologyNucleic acids and protein synthesis9700/23 · Oct/Nov 2022 · Q112 Marks

During translation, a polypeptide is synthesised when amino acids are added to a growing chain of amino acids. Fig. 1.1 shows part of a growing chain of amino acids and the amino acid cysteine. [Figure 1.1] Fig. 1.2 is a ribbon diagram showing the three-dimensional structure of a protein from the bacterium Streptococcus. [Figure 1.2]

  1. (a)(i)Complete Fig. 1.1 by showing the formation of the bond between cysteine and the growing chain of amino acids in the process of translation.[3]
  2. (a)(ii)State the name of the covalent bond that forms when cysteine is added to the growing chain of amino acids.[1]
  3. (a)(iii)State the organelle where the reaction shown in Fig. 1.1 takes place.[1]
  4. (b)(i)Describe the secondary structure of the protein shown in Fig. 1.2.[2]
  5. (b)(ii)Explain why the protein shown in Fig. 1.2 has tertiary structure, but not quaternary structure.[2]
  6. (b)(iii)An analysis of the amino acid composition of the protein in Fig. 1.2 showed that it does not contain any cysteine residues. Explain how the three-dimensional structure of the protein shown in Fig. 1.2 is held in place.[3]

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Mark scheme

Marking points from the official mark scheme (Oct/Nov 2022). The mark or mark type is shown in brackets.

  1. [1] C-N bond shown ;
  2. [1] =O shown on C and -H shown on N of peptide group ;
  3. [1] water shown as a product ;
  4. [1] peptide ;
  5. [1] ribosome ;
  6. [1] alpha- / α-, helix, helices ;
  7. [1] beta-/ẞ-, pleated sheet(s) / pleat(s) ;
  8. [1] region of no fixed shape / AW ;
  9. [1] protein is, a single polypeptide / made of one chain of amino acids
  10. [1] interactions / bonds, between, R-groups / side chains, (of amino acids) on the same polypeptide
  11. [1] in alpha-helix each N-H forms hydrogen bond with C=O three or four amino acids apart in same polypeptide ;
  12. [1] in beta-pleated sheet each N-H forms hydrogen bond with C=O on adjacent part of the polypeptide ;
  13. [1] hydrogen bonds between, R-groups with C=O and HN groups / carboxyl and amine groups ;
  14. [1] ionic bonds between R-groups with NH3+ and COO- groups ;
  15. [1] hydrophobic interactions between non-polar R-groups ;

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About this question

Topic
Nucleic acids and protein synthesis, A-Level Biology (syllabus 9700). Structured question, 12 marks.
Source
A-Level Biology, Oct/Nov 2022 Paper 2 Variant 3, question 1.

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