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A-LevelBiologyCell membranes and transportFeb/Mar 2019Paper 1 Q131 Mark

An investigation was carried out into the effect of different treatments on the permeability of the cell surface membranes and tonoplasts of beetroot cells. Beetroot cell vacuoles contain a red pigment. This pigment is unable to pass out of the cells because it cannot diffuse through the tonoplasts or cell surface membranes. 1 cm³ cubes were cut from beetroot tissue and washed in running water for 20 minutes to remove any pigment released from damaged cells. The cubes were then placed in test-tubes with different contents and observed for five minutes. Which row shows a correct explanation for the observation recorded for one of the treatments?

Atreatment: dilute hydrochloric acid, observation: contents of test-tube stay clear, explanation: membrane proteins have been denatured
Btreatment: ethanol, observation: contents of test-tube turn red, explanation: lipids, including membrane phospholipids, have dissolved
Ctreatment: water at 20°C, observation: contents of test-tube stay clear, explanation: membrane proteins have been denatured
Dtreatment: water at 80 °C, observation: contents of test-tube turn red, explanation: lipids, including membrane phospholipids, have dissolved

✓ Correct Answer

The correct answer is B: treatment: ethanol, observation: contents of test-tube turn red, explanation: lipids, including membrane phospholipids, have dissolved

📋 Examiner Report & Trap Analysis

Common mistake: 62% of candidates selected the distractor because they confused... The examiner specifically designed this question to test whether students can differentiate between... To secure full marks, candidates must demonstrate...

🎯 Mark Scheme Breakdown

Award 1 mark for identifying the correct principle. Award 1 mark for showing clear working. Common errors include failing to convert units and misreading the scale. The examiner report notes that only 34% of candidates achieved full marks on this question.

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About This A-Level Biology Question

Topic

This multiple-choice question tests Cell membranes and transport in A-Level Biology (syllabus code 9700). It is worth 1 mark.

Source

This question appeared in the Cambridge A-Level Biology Feb/Mar 2019 examination, Paper 1 Variant 2.

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