Phosphatidate phosphatase (PAP) enzymes have an important role in lipid metabolism. The reaction catalysed by PAP is shown in Fig. 2.1. phosphatidate + H2O PAP diglyceride + inorganic phosphate (Pi) Fig. 2.1 Experiments were carried out to investigate the activity of PAP extracted from the cotyledons (seed leaves) of bitter gourd, Momordica charantia.
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