Catechol is a chemical found in a number of fruits. Catechol can be oxidised to a quinone by the enzyme catechol oxidase. Catechol oxidase is inhibited by parahydroxybenzoic acid (PHBA), which is structurally similar to catechol. In the presence of PHBA, the Michaelis-Menten constant (Km) for catechol oxidase increases. How does PHBA inhibit catechol oxidase? 1 PHBA and catechol can both bind to the enzyme but not at the same time. 2 PHBA is a non-competitive inhibitor of catechol oxidase. 3 PHBA decreases the Vmax of the reaction.
✓ Correct answer
B: 1 only
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