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A-LevelBiologyEnzymes9700/12 · Feb/Mar 2021 · Q131 Mark

Catechol is a chemical found in a number of fruits. Catechol can be oxidised to a quinone by the enzyme catechol oxidase. Catechol oxidase is inhibited by parahydroxybenzoic acid (PHBA), which is structurally similar to catechol. In the presence of PHBA, the Michaelis-Menten constant (Km) for catechol oxidase increases. How does PHBA inhibit catechol oxidase? 1 PHBA and catechol can both bind to the enzyme but not at the same time. 2 PHBA is a non-competitive inhibitor of catechol oxidase. 3 PHBA decreases the Vmax of the reaction.

A1, 2 and 3
B1 only
C2 only
D3 only

✓ Correct answer

B: 1 only

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About this question

Topic
Enzymes, A-Level Biology (syllabus 9700). Multiple choice, 1 mark.
Source
A-Level Biology, Feb/Mar 2021 Paper 1 Variant 2, question 13.

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