A-LevelBiologyEnzymes9700/12 · Oct/Nov 2017 · Q151 Mark
An experiment was carried out in which the enzyme lipase was used to hydrolyse a triglyceride. The pH was recorded at regular intervals during the reaction. The results are shown in the table. [Figure 15.1 - table of pH changes over time during lipase reaction] At 14 minutes unreacted triglyceride was still present. What explains the results after 10 minutes?
This question uses a diagram from the original paper, which isn't shown here. Open the full paper from the past papers archive to see it.
AThe end-products acted as competitive inhibitors.
BThe end-products acted as non-competitive inhibitors.
CThe enzyme reaction had reached Vmax.
DThe tertiary structure of the enzyme had been lost.
✓ Correct answer
D: The tertiary structure of the enzyme had been lost.
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