A-LevelBiologyEnzymes9700/12 · Oct/Nov 2011 · Q131 Mark
Following a heart attack, the enzyme lactate dehydrogenase leaks into the blood plasma from damaged heart muscle. Which steps are required to obtain the best estimate of lactate dehydrogenase activity in a sample of blood plasma? [Table 13]
Asterilise blood plasma by heating: ✓
incubate with substrate for lactate dehydrogenase: ✓
incubate with lactate dehydrogenase inhibitor: ✓
Bsterilise blood plasma by heating: x
incubate with substrate for lactate dehydrogenase: ✓
incubate with lactate dehydrogenase inhibitor: x
Csterilise blood plasma by heating: x
incubate with substrate for lactate dehydrogenase: ✓
incubate with lactate dehydrogenase inhibitor: ✓
Dsterilise blood plasma by heating: x
incubate with substrate for lactate dehydrogenase: x
incubate with lactate dehydrogenase inhibitor: ✓
✓ Correct answer
C: sterilise blood plasma by heating: x incubate with substrate for lactate dehydrogenase: ✓ incubate with lactate dehydrogenase inhibitor: ✓
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