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A-LevelBiologyEnzymes9700/12 · Feb/Mar 2024 · Q141 Mark

A student investigated the hydrolysis of lipid in high-fat milk, using the enzyme lipase. • 1 cm³ of enzyme solution was added to 10 cm³ of high-fat milk. • The temperature was kept constant. The pH of the reaction mixture was recorded at time 0 minutes and every minute for 20 minutes. Which statements correctly describe the expected results of this investigation? 1 The product forms more slowly as time proceeds because the concentration of the substrate is decreasing. 2 The pH of the reaction mixture increases rapidly in the first few minutes and then increases less rapidly. 3 The increase in the concentration of product eventually causes the lipase molecules to denature.

A1, 2 and 3
B1 and 2 only
C1 and 3 only
D2 and 3 only

✓ Correct answer

C: 1 and 3 only

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About this question

Topic
Enzymes, A-Level Biology (syllabus 9700). Multiple choice, 1 mark.
Source
A-Level Biology, Feb/Mar 2024 Paper 1 Variant 2, question 14.

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