A student investigated the hydrolysis of lipid in high-fat milk, using the enzyme lipase. • 1 cm³ of enzyme solution was added to 10 cm³ of high-fat milk. • The temperature was kept constant. The pH of the reaction mixture was recorded at time 0 minutes and every minute for 20 minutes. Which statements correctly describe the expected results of this investigation? 1 The product forms more slowly as time proceeds because the concentration of the substrate is decreasing. 2 The pH of the reaction mixture increases rapidly in the first few minutes and then increases less rapidly. 3 The increase in the concentration of product eventually causes the lipase molecules to denature.
✓ Correct answer
C: 1 and 3 only
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