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A-LevelBiologyEnzymes9700/21 · May/June 2025 · Q17 Marks

Amylose and the triglyceride stearin are macromolecules. Students used the enzyme maltase extracted from the fungus Aspergillus oryzae to investigate the properties of enzymes. Fig. 1.1 is a diagram of a maltose molecule. [Figure 1.1]

  1. (a)Explain why amylose and stearin are macromolecules, but only amylose is a polymer.[2]
  2. (b)(i)Complete Fig. 1.1 to show the reaction catalysed by maltase.[3]
  3. (b)(ii)State the type of covalent bond that is broken in the reaction.[1]
  4. (b)(iii)State the type of reaction catalysed by maltase.[1]

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Mark scheme

Marking points from the official mark scheme (May/June 2025). The mark or mark type is shown in brackets.

  1. [1] (a) macromolecule (both are) large / AW, molecules / size / (molecular) mass ; A composed of, many / AW, atoms I composed of more than one molecule
  2. [1] (a) polymer composed of, many / three or more / more than two / repeated / repeating, subunits / units / monomers / residues / alpha glucose; A many of the same / similar / chain of for repeated I molecules
  3. [1] (b)(i) a-/alpha-, glucose molecule on left with –OH on C1 facing downwards ;
  4. [1] (b)(i) α-/alpha-, glucose molecule on right with –OH on C4 facing downwards ; ecf if a single same mistake is made in both, e.g. no –H on C5
  5. [1] (b)(i) involvement of water ; must be above or alongside arrow R if below
  6. [1] (b)(ii) glycosidic; A glucosidic I incorrect detail of the bond, e.g. ẞ-/beta- / α- / alpha-,1, 6
  7. [1] (b)(iii) hydrolysis ;

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About this question

Topic
Enzymes, A-Level Biology (syllabus 9700). Structured question, 7 marks.
Source
A-Level Biology, May/June 2025 Paper 2 Variant 1, question 1.

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