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A-LevelBiologyPhotosynthesis9700/43 · May/June 2023 · Q812 Marks

(a) Fig. 8.1 is a diagram of a chloroplast. K L M Fig. 8.1 Using the letters K–N, complete Table 8.1. Each letter may be used once, more than once, or not at all. Table 8.1 high concentration of protons location of photosynthetic pigments site of light-independent stage site of light-dependent stage N letter

  1. (a)Using the letters K–N, complete Table 8.1. Each letter may be used once, more than once, or not at all. Table 8.1 high concentration of protons location of photosynthetic pigments site of light-independent stage site of light-dependent stage letter[4]
  2. (b)Chlorophyll a is the main photosynthetic pigment in plants. Describe the role of other photosynthetic pigments found in plant chloroplasts.[4]
  3. (c)The absorption of different wavelengths of light by chloroplast pigments can be represented by an absorption spectrum. Fig. 8.2 is an absorption spectrum of an extract of chloroplast pigments and an absorption spectrum of whole chloroplasts. 10 9 8 7- 6 absorbance / arbitrary units 5 4 3 whole chloroplasts 2 pigment extract 1 0+ 400 450 500 550 600 650 700 wavelength/nm Fig. 8.2 With reference to Fig. 8.2, describe the differences between the two spectra and suggest explanations for the differences.[4]

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Mark scheme

Marking points from the official mark scheme (May/June 2023). The mark or mark type is shown in brackets.

  1. [1] high concentration of protons | M ;
  2. [1] location of photosynthetic pigments | L or N ;
  3. [1] site of light-independent stage | K ;
  4. [1] site of light-dependent stage | L or N ;
  5. ignore M in the last row
  6. any four from:
  7. [1] any one named; e.g. chlorophyll b / carotene / xanthophyll / carotenoids ;
  8. [1] act as accessory pigments / part of antenna complex / part of light harvesting system ;
  9. [1] absorb, light/ photons ;
  10. [1] pass energy on to, chlorophyll a / primary pigment / reaction centre ;
  11. [1] absorb different wavelengths of light / wavelengths not absorbed by chlorophyll a ;
  12. [1] absorption higher for (whole) chloroplasts (throughout) ;
  13. [1] comparative data quote or greatest difference at 525 / 530 nm ;
  14. wavelength / nm | absorbance / au ±0.05 | whole | pigment
  15. 500 | 9.2 | 6.4
  16. 510 | 8.4 | 3.8
  17. 520 | 7.4 | 2.3
  18. 525 | 7.0 | 1.95
  19. 530 | 6.6 | 1.85
  20. 540 | 6.2 | 2.05
  21. 550 | 5.8 | 2.35
  22. 560 | 5.8 | 2.7
  23. 600 | 6.65 | 4.7
  24. 650 | 8.85 | 8.1
  25. 670 | 9.6 | 9.2
  26. [1] (because) pigments arranged for better absorption in chloroplasts / thylakoid membranes are stacked / AW ;
  27. [1] (because) chloroplasts contain more pigments ;

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About this question

Topic
Photosynthesis, A-Level Biology (syllabus 9700). Structured question, 12 marks.
Source
A-Level Biology, May/June 2023 Paper 4 Variant 3, question 8.

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