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A-LevelBiologyPhotosynthesis9700/41 · May/June 2017 · Q29 Marks

Corals grow in shallow seawater. Corals consist of colonies of small animals called polyps. These polyps have photosynthetic protoctists called algae inside their cells, which is advantageous both to the coral polyps and to the algae. The algae that live within the cells of the polyps can also live independently as free-living algae.

  1. (a)The rate of photosynthesis of algae that live within the cells of coral polyps is higher than that of free-living algae. Suggest and explain how living inside the cells of coral polyps increases the rate of photosynthesis in these algae compared to free-living algae.[2]
  2. (b)The relative abundance of five different chloroplast pigments in the algae of corals was determined. The results are shown in Table 2.1. Table 2.1 chloroplast pigment | percentage of total chlorophyll a | 39 peridinin | 39 chlorophyll c2 | 13 dinoxanthin | 7 ẞ-carotene | 2 Outline the method you would use to separate and identify the pigments present in an extract of these algae.[4]
  3. (c)Table 2.2 shows the light wavelengths at which each algal chloroplast pigment shows its two largest peaks of light absorption. Table 2.2 chloroplast pigment | peak 1 wavelength /nm | peak 2 wavelength /nm chlorophyll a | 430 | 662 peridinin | 456 | 485 chlorophyll c2 | 450 | 396 dinoxanthin | 442 | 471 ẞ-carotene | 454 | 480 Corals kept in tanks are often illuminated by lamps radiating mostly violet and blue light with wavelengths in the range of 400–490 nm. With reference to Table 2.1 and Table 2.2, suggest why lamps radiating mostly violet and blue light are expected to increase coral growth.[3]

Mark scheme

Marking points from the official mark scheme (May/June 2017). The mark or mark type is shown in brackets.

  1. 1 high / increased / better / not limiting, carbon dioxide / temperature / (named) mineral ions ; I nutrients
  2. 2 (from) coral/polyp, respiration / metabolism or for algae, Calvin cycle / light independent reactions; A correct use of mineral ions
  3. max 4 of:
  4. 1 (paper / thin layer) chromatography / chromatogram ;
  5. 2 place spot of, extract / pigments, on pencil mark / at base of, paper / TLC plate;
  6. 3 dry and repeat (to concentrate spot);
  7. 4 dip, paper/chromatogram, in solvent/so solvent travels up paper; A named organic solvent (I water) R if spot submerged
  8. 5 measure distance travelled by solvent (front) and pigment (spot);
  9. 6 (calculate) Rf value = distance travelled by pigment / distance travelled by solvent (front) ;
  10. 7 look up/compare results with, known Rf values (to identify pigments) ;
  11. max 3 of:
  12. 1 pigments absorb, violet-blue / 400–490 nm/lamp colours, well / best / most / at 8 out of 10 peaks ;
  13. 2 rate of photosynthesis of algae increases with more light absorbed ;
  14. 3 coral growth (increases) with more (algal) photosynthesis ; R products respond to give growth
  15. 4 chlorophyll a and peridinin are, most abundant pigments / most important;
  16. 5 AVP; e.g. violet-blue / 400–490 nm, predominate at the depths where corals live

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About this question

Topic
Photosynthesis, A-Level Biology (syllabus 9700). Structured question, 9 marks.
Source
A-Level Biology, May/June 2017 Paper 4 Variant 1, question 2.

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