Fig. 3.1 shows some fruits (grapes) of the grapevine, Vitis vinifera. Sucrose is transported in the phloem of the grapevine to the fruits. In the fruits, sucrose is hydrolysed by the enzyme sucrase, which is found in cell walls. The glucose and fructose produced by the hydrolysis of sucrose pass through membrane proteins, known as hexose transporters, into the cytoplasm of the fruit cells.
- (a)State why membrane proteins are required for the movement of molecules, such as glucose, across cell surface membranes into cells.[1]
- (b)(i)The researchers concluded that VvHT1 is responsible for the facilitated diffusion of glucose into the cells. Explain how the results in Fig. 3.2 provide evidence to support this conclusion.[2]
- (b)(ii)The researchers thought that grapevines could be modified to have more hexose transporters to increase the size and quality of grapes. Explain why increasing the number of hexose transporters could be commercially important to growers of grapevines.[2]
- (c)(i)State what is meant by a non-self antigen.[1]
- (c)(ii)Some pathogens enter human cells. Macrophages partially digest these pathogens and present antigens to T-lymphocytes during immune responses. With reference to Fig. 3.3, explain how T-lymphocytes respond to infection by a specific type of pathogen.[4]
This question uses a diagram from the original paper, which isn't shown here. Open the full paper from the past papers archive to see it.
Mark scheme
Marking points from the official mark scheme (May/June 2023). The mark or mark type is shown in brackets.
- [1] polar / water soluble / hydrophilic, substances cannot pass through the, phospholipid bilayer / hydrophobic core (in cell surface membranes); I too large / active transport / facilitated diffusion / (hydrophobic) fatty acid 'tails'
- [2] rate of uptake, becomes constant / reaches a plateau or at high concentrations / concentrations of > 150 µmol dm⁻³, rate of uptake remains constant; A levels off protein carriers / (hexose) transporters / VvHT1s, are working at their highest rate / are saturated ; A the number of protein carriers is limiting factor / AW A Vmax is reached in context of transporters
- [2] any two from: I size / quality of grapes / grapevines 1 grapes will, be sweeter / have improved taste ; 2 idea that concentration of sugars in grapes determines uptake of water by osmosis ; 3 idea of more energy for growth of the grapes ; 4 larger yield ; 5 increased, profit / income, for farmers ; 6 AVP; e.g. producing grapes in shorter time
- [1] (foreign) protein / glycoprotein, that stimulates, an immune response / production of antibodies / activation of lymphocytes ; A polysaccharide / molecule / foreign substance / foreign antigen
- [4] any four from: accept T-cells for T-lymphocytes 1 (group / clone of) T-lymphocytes have receptors complementary to an antigen ; A specific T-lymphocytes have receptors to an antigen 2 (specific) T-lymphocytes, bind / AW, to antigen (on surface of antigen-presenting cell / macrophage) ; 3 (selected) T-lymphocytes, divide many times (by mitosis) / undergo clonal expansion ; 4 T-helper cells secrete, cytokines / interleukins / interferon ; 5 detail of effect of cytokine ; e.g. activation of B-lymphocytes / increased phagocytosis by macrophages / 'angry' macrophages 6 T-killer cells, bind / attach, to infected cells and destroy them; 7 detail of action of T-killer cells ; e.g. ref. to, perforin / granzyme / hydrogen peroxide / toxins 8 production of memory cells (in context of T-lymphocytes / T-helper cells / T-killer cells);
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