Plant tissues contain the enzyme catalase which catalyses the breakdown of hydrogen peroxide into oxygen gas and water.
Ascorbic acid acts as an inhibitor of catalase.
You will investigate the effect of changing ascorbic acid concentration on catalase inhibition.
You are provided with the materials shown in Table 1.1.
Table 1.1
labelled contents hazard volume/cm³
H hydrogen peroxide solution harmful irritant 50
W distilled water none 50
A 1 mol dm-3 ascorbic acid solution irritant 10
If H or A comes into contact with your skin, wash off immediately under cold water.
It is recommended that you wear suitable eye protection.
You are also provided with five cylinders of potato tissue, labelled P.

Q: What topic does this question test?

This question tests Enzymes in A-Level Biology (syllabus code 9700). It is worth 22 marks.

Q: Which exam paper is this question from?

This question appeared in the Cambridge A-Level Biology May/June 2022 examination, Paper 3 Variant 2 (9700_s22_qp_32.pdf).

Question

Plant tissues contain the enzyme catalase which catalyses the breakdown of hydrogen peroxide into oxygen gas and water.
Ascorbic acid acts as an inhibitor of catalase.
You will investigate the effect of changing ascorbic acid concentration on catalase inhibition.
You are provided with the materials shown in Table 1.1.
Table 1.1
labelled	contents	hazard	volume/cm³
H	hydrogen peroxide solution	harmful irritant	50
W	distilled water	none	50
A	1 mol dm-3 ascorbic acid solution	irritant	10
If H or A comes into contact with your skin, wash off immediately under cold water.
It is recommended that you wear suitable eye protection.
You are also provided with five cylinders of potato tissue, labelled P.

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