O-LevelBiologyGenetics & Variation5090/12 · May/June 2021 · Q401 Mark

Some stages in the production of human insulin are listed. 1 Genetically modified E. coli bacteria are grown in large fermenters. 2 The gene for human insulin is inserted into the DNA of a bacterium called E. coli. 3 The gene for human insulin is obtained from human pancreas cells. 4 Human insulin is extracted and purified. What is the correct sequence of these stages?

A3→1→2→4

B4→3→2→1

C3→2→4→1

D3→2→1→4

✓ Correct answer

D: 3→2→1→4

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Topic: Genetics & Variation, O-Level Biology (syllabus 5090). Multiple choice, 1 mark.
Source: O-Level Biology, May/June 2021 Paper 1 Variant 2, question 40.

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