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O-LevelBiologyGenetics & Variation5090/22 · May/June 2016 · Q810 Marks

Describe the industrial manufacture of single cell protein.

  1. (a)Describe the industrial manufacture of single cell protein.[8]
  2. (b)Suggest problems of using viruses in biotechnology.[2]

Mark scheme

Marking points from the official mark scheme (May/June 2016). The mark or mark type is shown in brackets.

  1. [max 8] 1. sterilised / aseptic technique ;
  2. 2. fermenter ;
  3. 3. bacterium / fungus / algae / named example;
  4. 4. substrate / cultured medium or named / broth / any two chemicals in the medium;
  5. 5. oxygen / air;
  6. 6. bubbles / sparger / aerator / paddle / stirrer AW ;
  7. 7. temperature regulation / control / cooling jacket;
  8. 8. pH ref;
  9. 9. optimum or best for growth or reproduction of organism ;
  10. 10. filtration / collection / harvesting / separating (the product);
  11. 11. name / use of product e.g. mycoprotein / meat substitute / cattle feed;
  12. [max 2] 1. size reference/extremely small AW;
  13. 2. reproduce only in living cells/pathogenic AW/parasitic; A named viral disease
  14. 3. specific;
  15. 4. may need to separate from living tissue/difficult to isolate ;

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About this question

Topic
Genetics & Variation, O-Level Biology (syllabus 5090). Structured question, 10 marks.
Source
O-Level Biology, May/June 2016 Paper 2 Variant 2, question 8.

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