A student investigates the use of an enzyme in fruit juice production. The student carries out the following steps: • cuts one apple into small pieces • puts the same number of pieces into each of three beakers, A, B and C • adds 2cm³ of a dilute enzyme solution to the apple in beakers A and B • adds 2cm³ of distilled water to the apple in beaker C • places each beaker in a water-bath at a constant temperature for 30 minutes • filters the juice from each beaker into a separate measuring cylinder and records the volume of juice collected in each measuring cylinder.

1. (a)(i)Identify the enzyme that the student should add to the apple in beakers A and B.[1]
2. (a)(ii)Outline the reason why the student set up beaker C.[1]
3. (b)The results of the student's investigation are shown in Table 5.1. Table 5.1 beaker temperature of water-bath /°C volume of juice collected /cm³ A 45 20 B 90 5 C 45 5 Use your knowledge of enzyme action to explain these results.[5]
4. (c)Bacteria can be genetically modified to produce enzymes for use in biotechnology. State two reasons why bacteria are suitable for this purpose. 1 2[2]