The diagram shows a cross-section through a leaf when viewed using a light microscope. [Figure 2.1]

1. (a)(i)State the term used to describe a group of cells, such as those in part A or part B of the leaf cross-section.[1]
2. (a)(ii)The diagram shows an enlargement of one cell from part A of the leaf cross-section. [Figure 2.2] Complete the diagram of the cell by drawing and labelling to show the position of • one chloroplast • three other types of named cell component that will be visible.[4]
3. (a)(iii)Use a label line on the diagram of the cross-section through a leaf to name and label one cell in the lower epidermis that would also contain chloroplasts.[1]
4. (b)(i)The cell wall of a plant cell can be removed by treating the cell with a digestive enzyme. Name the substrate for this enzyme.[1]
5. (b)(ii)Some plant cells from part B of the leaf cross-section were treated with this enzyme and then placed in distilled water on a microscope slide for one hour. The cells were clearly visible using a light microscope at the start of the hour. The cells were not clearly visible using the same light microscope at the end of the hour. Explain changes to the structure of the cells that took place between these two observations.[4]

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