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O-LevelBiologyEnzymes5090/22 · Oct/Nov 2021 · Q511 Marks

Some laundry detergents used to wash clothes contain enzymes. These enzymes break down the molecules that cause stains.

  1. (a)Suggest enzymes that may be components of a laundry detergent that will break down stains made of: starch fat.[2]
  2. (b)(i)Savinase is a protease enzyme produced by genetically engineered bacteria. The enzyme is a component of laundry detergents. The graphs show the results of an investigation into the effects of temperature and pH on the rate of Savinase activity. [Figure 5.0] Describe how the effect of temperature on Savinase activity differs from the effect of temperature on a protease that functions in the human stomach.[3]
  3. (b)(ii)Suggest why laundry detergents that contain Savinase also contain chemicals that dissolve to form an alkaline solution.[2]
  4. (b)(iii)After washing clothes using laundry detergents, the waste water is sometimes released into the environment. This waste water contains inorganic phosphate ions that are also found in fertilisers. Explain the harmful effect on aquatic life of releasing this waste water into the environment.[4]

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Mark scheme

Marking points from the official mark scheme (Oct/Nov 2021). The mark or mark type is shown in brackets.

  1. [2] (starch) amylase ; (fat) lipase ;
  2. [3] optimum AW + 60°C ; (optimum) not 37°C ; correct ref. temperature + denaturation for either enzyme ;
  3. [2] savinase / enzyme + increased rate / works faster / optimum ; prevents denaturation ; detergent more effective / clothes cleaner ;
  4. [4] pollution ; eutrophication ; two correct details of the eutrophication process for two marks ;; less oxygen available / kills + fish / consumers / animals AW ;

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About this question

Topic
Enzymes, O-Level Biology (syllabus 5090). Structured question, 11 marks.
Source
O-Level Biology, Oct/Nov 2021 Paper 2 Variant 2, question 5.

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