Paper chromatography can be used to separate the individual amino acids formed when tripeptides are hydrolysed. One molecule of a tripeptide produces three amino acid molecules when hydrolysed. A student is asked to identify the amino acids formed from the hydrolysis of three different tripeptides, A, B and C, using paper chromatography. [Figure 2.1] shows the results of the student's chromatography experiment. [Figure 2.1] The individual amino acids can be identified from their R_f values. R_f = distance travelled by the amino acid spot / distance travelled by the solvent front