A student investigated the hydrolysis of the lipid in high-fat milk, using the enzyme lipase. • 1 cm³ of enzyme solution was added to 10 cm³ of high-fat milk. • The temperature was kept constant. • The pH of the reaction mixture was recorded at time 0 minutes and every minute for 20 minutes. Which statements could be supported by the results of the investigation? 1 Less product is made as time proceeds because the substrate is decreasing. 2 The pH of the reaction mixture changes more rapidly in the first few minutes and then changes less rapidly. 3 The product gradually causes more lipase molecules to denature.