A student investigated the hydrolysis of the lipid in high-fat milk, using the enzyme lipase.
• 1 cm³ of enzyme solution was added to 10 cm³ of high-fat milk.
• The temperature was kept constant.
• The pH of the reaction mixture was recorded at time 0 minutes and every minute for 20 minutes.
Which statements could be supported by the results of the investigation?
1 Less product is made as time proceeds because the substrate is decreasing.
2 The pH of the reaction mixture changes more rapidly in the first few minutes and then changes less rapidly.
3 The product gradually causes more lipase molecules to denature.

Q: What topic does this question test?

This question tests Enzymes in A-Level Biology (syllabus code 9700). It is worth 1 mark.

Q: Which exam paper is this question from?

This question appeared in the Cambridge A-Level Biology May/June 2018 examination, Paper 1 Variant 2 (9700_s18_qp_12.pdf).

Question

A student investigated the hydrolysis of the lipid in high-fat milk, using the enzyme lipase.
• 1 cm³ of enzyme solution was added to 10 cm³ of high-fat milk.
• The temperature was kept constant.
• The pH of the reaction mixture was recorded at time 0 minutes and every minute for 20 minutes.
Which statements could be supported by the results of the investigation?
1 Less product is made as time proceeds because the substrate is decreasing.
2 The pH of the reaction mixture changes more rapidly in the first few minutes and then changes less rapidly.
3 The product gradually causes more lipase molecules to denature.

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The correct answer is A: 1, 2 and 3.

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