P1 is a slide of a stained transverse section through a plant leaf. (b) Fig. 2.1 is a photomicrograph of a transverse section through a leaf of a different species of plant. [Figure 2.1] (c) Fig. 2.2 is the same photomicrograph of a transverse section of a leaf as is shown in Fig. 2.1. [Figure 2.2]

1. (a)(i)Draw a large plan diagram of part of the leaf section on P1 to show all of the different tissues. The part of the section that you draw should show the full depth of the leaf section from the upper surface to the lower surface and must include at least one vascular bundle. Use one ruled label line and label to identify the epidermis.[5]
2. (a)(ii)Observe the epidermal cells of the leaf on P1. Select four adjacent epidermal cells that are arranged in a line. Each cell must touch at least one other cell. • Make a large drawing of this line of four cells. • Use one ruled label line and label to identify the cell wall.[5]
3. (b)(i)Identify three observable features, other than colour, that are different between the leaf in Fig. 2.1 and the leaf on P1. Record the differences between these three observable features in Table 2.1. Table 2.1 feature Fig. 2.1 P1[3]
4. (b)(ii)The leaf section shown in Fig. 2.1 is from a plant that is adapted to live in water. State one feature visible in Fig. 2.1 that adapts the plant to live in water. Suggest the function of this feature. feature ................................................................ function ................................................................[1]
5. (c)(i)In Fig. 2.2, the lines L1, L2, L3 and L4 are drawn across the lengths of four air spaces. Measure the lengths in the photomicrograph of these four air spaces, along the lines L1, L2, L3 and L4. length of L1 = .............................................. length of L2 = .............................................. length of L3 = .............................................. length of L4 = .............................................. Calculate the mean length in the photomicrograph of the four air spaces. Show your working. mean length = ..............................................[2]
6. (c)(ii)Calculate the mean actual length of these four air spaces in micrometres (µm), using your answer to (c)(i) and the magnification in Fig. 2.2. Show your working. Give your answer to an appropriate number of significant figures. mean actual length = .............................................. µm[2]

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