Plant cells produce the enzyme catalase that catalyses the hydrolysis (breakdown) of hydrogen peroxide into water and oxygen, as shown in Fig. 1.1. catalase hydrogen peroxide ---> water + oxygen [Figure 1.1] A cylinder of potato tissue will have catalase molecules on its surface. When potato tissue is put into hydrogen peroxide solution, oxygen bubbles are released and a foam forms at the surface of the hydrogen peroxide solution. You will investigate the effect of ethanol concentration on the activity of catalase by measuring the height of the foam. You are provided with the materials shown in Table 1.1. Table 1.1 labelled | contents | hazard | volume/cm³ H | hydrogen peroxide solution | harmful irritant | 40 W | water | none | 200 E | 100% ethanol | flammable | 80 P | 5 potato cylinders | none | – If any of H comes into contact with your skin, wash off immediately with cold water. It is recommended that you wear suitable eye protection.
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