During transcription, base pairing occurs between nucleotides. Fig. 3.1 is a diagram to show complementary base pairing between a DNA nucleotide and an RNA nucleotide. Only the base pair is shown in molecular detail. [Figure 3.1]
- (a)Explain why Fig. 3.1 does not include any phosphodiester bonds.[1]
- (b)Identify and describe the DNA-RNA nucleotide pair shown in Fig. 3.1. You may add labels and annotations to Fig. 3.1 if you wish.[4]
This question uses a diagram from the original paper, which isn't shown here. Open the full paper from the past papers archive to see it.
Mark scheme
Marking points from the official mark scheme (Oct/Nov 2024). The mark or mark type is shown in brackets.
- [1] do not allow 'base' as AW for 'nucleotide' any one from: idea that phosphodiester bond forms, between nucleotides on the same strand / between adjacent nucleotides / during polynucleotide formation / to form a sugar-phosphate backbone; AW look for understanding that more than one nucleotide needs to be present on the same strand idea that phosphates, shown are only bound to one (pentose) sugars / each phosphate needs to be bound to two (pentose) sugars;
- [4] base / nucleotide, on left identified as guanine and, base / nucleotide, on right identified as cytosine ; A guanine and cytosine as question has DNA-RNA I cytosine and guanine unless further qualified dotted line as hydrogen bond / annotation related to dotted lines as hydrogen bonds / there are three hydrogen bonds between the bases; A H-bond circle is phosphate; pentagon/pentose, on left is deoxyribose ; also AVP if 'pentose' double ring/ base on left, identified as purine ; A guanine is a purine if mp1 gained single ring / base on right, identified as pyrimidine ; A cytosine is a pyrimidine if mp1 gained AVP; e.g. label to any solid line bond as covalent bond phospho-ester bond, labelled / described pentose sugar, label to / described for, deoxyribose / ribose box drawn around one completed nucleotide and labelled 5' and 3' shown annotated ref. to antiparallel nature of base-pair
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