A-LevelBiologyEnzymes9700/12 · Oct/Nov 2023 · Q141 Mark
Which statements about the Michaelis–Menten constant (Km) of an enzyme are correct? 1 At the Km value, half the active sites of the enzyme should be occupied by the substrate. 2 Km represents the substrate concentration at which the enzyme is working at half its maximum rate. 3 The lower the Km value, the lower the affinity of the enzyme to its substrate. 4 When an enzyme has a high Km value, the enzyme-catalysed reaction will proceed very slowly to its maximum rate.
A1, 2 and 3
B1, 2 and 4
C1 and 3 only
D2 and 4 only
✓ Correct answer
B: 1, 2 and 4
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