A-LevelBiologyEnergy and respirationFeb/Mar 2019Paper 4 Q79 Marks
Isolated mitochondria were used to investigate respiration. • Mitochondria were extracted from respiring mammalian cells and incubated in a buffer solution. • Pyruvate and inorganic phosphate (Pi) were added at time zero. • ADP was added one minute later. • The oxygen concentration of the buffer solution containing mitochondria was monitored throughout the investigation. The results of the investigation are shown in [Figure 7.1].
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