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A-LevelBiologyNucleic acids and protein synthesisOct/Nov 2010Paper 1 Q211 Mark

A culture of bacteria had all its DNA labelled with the heavy isotope of nitrogen (15N). A sample was taken and spun in a centrifuge. The diagram shows the position of the DNA band at Z in the centrifuge tube. [Figure 21.1 showing a centrifuge tube with bands labeled X, Y, Z] The culture was then allowed to reproduce using nucleotides containing the normal isotope of nitrogen (14N). Samples were taken and spun in a centrifuge after one generation and again after two generations. In which pattern would the DNA be found after the first and after the second generations? after first generation after second generation

Ahalf at X and half at Y quarter at X, quarter at Z and half at Y
Bhalf at X and half at Z quarter at X, quarter at Z and half at Y
Call at Y half at X and half at Y
Dall at Z half at Y and half at Z

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The correct answer is C. This question tests the candidate's understanding of nucleic acids and protein synthesis within the Biologysyllabus. The examiner's mark scheme requires...

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About This A-Level Biology Question

This multiple-choice question appeared in the Cambridge A-Level Biology (9700) Oct/Nov 2010 examination, Paper 1 Variant 2. It tests the topic of Nucleic acids and protein synthesis and is worth 1 mark.

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