A chloroplast is composed of many structures, each with a different function. Several chloroplast structures are listed. stroma lamellae thylakoid membrane ribosome starch grain DNA outer membrane thylakoid space From the list:
- (a)(i)identify the structures involved in the production of rubisco[2]
- (a)(ii)identify the structure that contains a high concentration of protons in daylight.[1]
- (b)Paper chromatography is a technique that can be used to separate a mixture of four common chloroplast pigments. The pigments can be identified by calculating their R₁ values. A student carried out paper chromatography on a solution containing a mixture of chloroplast pigments. The results are shown in Table 7.1. [Table 7.1] Complete Table 7.1.[3]
- (c)Fig. 7.1 shows the absorption spectrum for carotene and for chlorophyll a. [Figure 7.1] With reference to Fig. 7.1, describe and explain the role of carotene in photosynthesis.[4]
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Mark scheme
Marking points from the official mark scheme (May/June 2025). The mark or mark type is shown in brackets.
- [2] 7(a)(i) DNA; ribosome;
- [1] 7(a)(ii) thylakoid space;
- [3] 7(b) xanthophyll; 3.4; 0.92;
- [4] 7(c) any four from: 1 absorbs light between 400 and 500 nm / peak at 450 nm ; 2 accessory pigment; 3 absorbs light wavelengths not absorbed by, reaction centre / primary pigment / chlorophyll a ; A harvest 4 so extends the range of wavelengths absorbed ; 5 pass energy to, reaction centre / primary pigment / chlorophyll a ; 6 idea improves efficiency of light-dependent stage ;
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