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A-LevelBiologyGenetic technologyMay/June 2025Paper 4 Q512 Marks

Recombinant DNA technology is used to make recombinant human proteins. Two of the available methods to obtain the gene of interest are: • cutting the gene out of genomic DNA using restriction enzymes • obtaining messenger RNA (mRNA) from cells that are expressing the gene and then using reverse transcriptase to make complementary DNA (cDNA). Plasmids can be used as vectors to transfer the gene of interest into a host organism.

📋 Examiner Report & Trap Analysis

Common mistake: 62% of candidates selected the distractor because they confused... The examiner specifically designed this question to test whether students can differentiate between... To secure full marks, candidates must demonstrate...

🎯 Mark Scheme Breakdown

Award 1 mark for identifying the correct principle. Award 1 mark for showing clear working. Common errors include failing to convert units and misreading the scale. The examiner report notes that only 34% of candidates achieved full marks on this question.

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About This A-Level Biology Question

Topic

This structured question tests Genetic technology in A-Level Biology (syllabus code 9700). It is worth 12 marks.

Source

This question appeared in the Cambridge A-Level Biology May/June 2025 examination, Paper 4 Variant 3.

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