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A-LevelBiologyEnergy and respiration9700/41 · May/June 2025 · Q25 Marks

A respirometer can be used to investigate how temperature affects the rate of respiration of woodlice. Woodlice are small invertebrate animals. Fig. 2.1 shows a single woodlouse. [Figure 2.1] Fig. 2.2 shows a simple respirometer. [Figure 2.2]

  1. (a)Explain how the experimental set-up in Fig. 2.2 allows the rate of respiration of woodlice to be determined.[4]
  2. (b)A student determined the rate of respiration of woodlice at a temperature of 5°C and at a temperature of 20 °C. Predict and explain how the rate of respiration of woodlice differs at 5°C compared to 20 °C.[1]

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Mark scheme

Marking points from the official mark scheme (May/June 2025). The mark or mark type is shown in brackets.

  1. [4] (a) any four from: 1 sodium hydroxide absorbs, carbon dioxide / CO2, released by, woodlice / respiration ; 2 oxygen / O2, taken in reduces pressure (in boiling tube); 3 drop of liquid moves towards, boiling tube / bung / woodlice / left; 4 measure, distance / length, moved by drop, in set time; 5 rate = distance divided by time; 6 detail of calculation of volume of O2 used;
  2. [1] (b) rate at 5 °C: lower/slower and less/lower, kinetic energy or fewer enzyme-substrate, collisions / complexes or fewer, enzyme-catalysed / respiration / glycolysis / link reaction / Krebs cycle, reactions ;

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About this question

Topic
Energy and respiration, A-Level Biology (syllabus 9700). Structured question, 5 marks.
Source
A-Level Biology, May/June 2025 Paper 4 Variant 1, question 2.

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