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A-LevelBiologyEnergy and respiration9700/41 · May/June 2025 · Q111 Marks

Organisms need a source of energy for many cellular processes. Respiration involves the release of energy from energy-rich molecules for the synthesis of ATP molecules. ATP is described as the energy currency of cells.

  1. (a)Name two energy-rich molecules that enter the respiration pathway to synthesise ATP.[2]
  2. (b)Fig. 1.1 shows a molecule of ATP. [Figure 1.1] Explain the features of ATP that make it suitable to be the universal energy currency of cells.[4]
  3. (c)Identify the type of phosphorylation reaction to synthesise ATP that occurs during glycolysis and the Krebs cycle.[1]
  4. (d)(i)Pyruvate moves into the matrix of the mitochondrion only when a particular inorganic molecule is present. Name the inorganic molecule that must be present in the cell for pyruvate to enter the matrix of the mitochondrion.[1]
  5. (d)(ii)Explain how the presence of the inorganic molecule named in (d)(i) affects the ATP yield from respiration.[3]

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Mark scheme

Marking points from the official mark scheme (May/June 2025). The mark or mark type is shown in brackets.

  1. [2] (a) any two from: glucose (phosphate); fructose (phosphate / bisphosphate); triose phosphate ; (named) fatty acid / keto-acid; glycerol; amino acid;
  2. [4] (b) any four from: 1 breaks down / hydrolyses to, ADP / AMP, and Pi; 2 releases, energy / 30.5 kJ mol-1 ; 3 reversible reaction / high turnover / can be regenerated ; 4 small/soluble, so can, diffuse / move (freely), in cell ; 5 AVP;
  3. [1] (c) substrate-linked / substrate-level (phosphorylation) ;
  4. [1] (d)(i) oxygen;
  5. [3] (d)(ii) presence of oxygen: 1 (ATP yield) increases / is higher ; plus any two from: 2 oxygen acts as final, electron / proton, acceptor; 3 oxidative phosphorylation / chemiosmosis (occurs); 4 energy from, reduced NAD / reduced FAD / electron transport chain ; 5 proton gradient / H+ → into intermembrane space ; 6 ATP made in Krebs cycle;

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About this question

Topic
Energy and respiration, A-Level Biology (syllabus 9700). Structured question, 11 marks.
Source
A-Level Biology, May/June 2025 Paper 4 Variant 1, question 1.

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