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A-LevelBiologyEnzymesMay/June 2025Paper 2 Q39 Marks

Scientists investigated the progress of reactions catalysed by two enzymes: dopa oxidase and neutrase. The reactions catalysed by these enzymes result in changes to the appearance of the reaction mixtures. The reactions are shown in Fig. 3.1. The changes in appearance of the reaction mixtures make it possible to follow the reactions using a colorimeter. Fig. 3.2 shows the progress of the reaction catalysed by dopa oxidase as recorded from a colorimeter. Fig. 3.3 shows the progress of the reaction catalysed by neutrase as recorded from a colorimeter. Scientists searching for a suitable enzyme to use in an industrial process isolated the bacterium Vibrio parahaemolyticus from the mouth of the Mediterranean eel, Muraena helena. The scientists discovered an enzyme in the bacterium that was suitable for the industrial process. The scientists named the enzyme VpSP37. The scientists investigated how the rate of reaction catalysed by VpSP37 is affected by the concentration of its substrate. The results of the investigation are shown in Fig. 3.4. [Figure 3.1] [Figure 3.2] [Figure 3.3] [Figure 3.4]

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About This A-Level Biology Question

This structured question appeared in the Cambridge A-Level Biology (9700) May/June 2025 examination, Paper 2 Variant 1. It tests the topic of Enzymes and is worth 9 marks.

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