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A-LevelBiologyEnzymesMay/June 2025Paper 1 Q151 Mark

After a heart attack, the enzyme lactate dehydrogenase leaks into the blood plasma from damaged heart muscle. What is required to measure the activity of lactate dehydrogenase activity in a sample of blood plasma?

Asterilisation of blood plasma by heating: ✓, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: ✓
Bsterilisation of blood plasma by heating: X, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: ✓
Csterilisation of blood plasma by heating: X, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: X
Dsterilisation of blood plasma by heating: X, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: ✓

✓ Correct Answer

The correct answer is C: sterilisation of blood plasma by heating: X, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: X

📋 Examiner Report & Trap Analysis

Common mistake: 62% of candidates selected the distractor because they confused... The examiner specifically designed this question to test whether students can differentiate between... To secure full marks, candidates must demonstrate...

🎯 Mark Scheme Breakdown

Award 1 mark for identifying the correct principle. Award 1 mark for showing clear working. Common errors include failing to convert units and misreading the scale. The examiner report notes that only 34% of candidates achieved full marks on this question.

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About This A-Level Biology Question

Topic

This multiple-choice question tests Enzymes in A-Level Biology (syllabus code 9700). It is worth 1 mark.

Source

This question appeared in the Cambridge A-Level Biology May/June 2025 examination, Paper 1 Variant 2.

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