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A-LevelBiologyEnzymes9700/12 · May/June 2025 · Q151 Mark

After a heart attack, the enzyme lactate dehydrogenase leaks into the blood plasma from damaged heart muscle. What is required to measure the activity of lactate dehydrogenase activity in a sample of blood plasma?

Asterilisation of blood plasma by heating: ✓, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: ✓
Bsterilisation of blood plasma by heating: X, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: ✓
Csterilisation of blood plasma by heating: X, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: X
Dsterilisation of blood plasma by heating: X, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: ✓

✓ Correct answer

C: sterilisation of blood plasma by heating: X, incubation of sample with substrate for lactate dehydrogenase: ✓, incubation of sample with lactate dehydrogenase inhibitor: X

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About this question

Topic
Enzymes, A-Level Biology (syllabus 9700). Multiple choice, 1 mark.
Source
A-Level Biology, May/June 2025 Paper 1 Variant 2, question 15.

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