An investigation was carried out to study the effect of different intensities of blue light on the percentage germination of barley seeds. Barley seeds were exposed to blue light for a period of seven days. All other variables were kept constant. The results are shown in Table 9.1. Table 9.1 light intensity /arbitrary units (au): 0 (dark), 36, 48, 57. percentage germination: 98.0, 76.9, 45.0, 14.7. The effect of blue light on the concentration of abscisic acid (ABA) was also investigated. ABA concentration was measured at intervals over seven days in barley seeds exposed to blue light at an intensity of 57 arbitrary units. The results are shown in Table 9.2. Table 9.2 day: 0, 1, 3, 5, 7. concentration of ABA /arbitrary units (au): 100, 90, 350, 351, 381. For comparison, in the dark the concentration of ABA in barley seeds fell from 100 au at the start (day 0) to 45 au on day 1 and did not increase from day 1 to day 7.
- (a)Using the information in Table 9.1 and Table 9.2, describe the effect of blue light on the germination of barley seeds and suggest an explanation for this effect.[4]
- (b)After germination, auxin is important in the growth of barley plants. Describe and explain the role of auxin in cell elongation.[7]
Mark scheme
Marking points from the official mark scheme (Feb/Mar 2024). The mark or mark type is shown in brackets.
- [1] (blue) light decreases germination / AW ;
- [1] as intensity of (blue) light increases percentage germination decreases ;
- [1] data quote ;
- [1] (blue) light increases (the concentration of) ABA ;
- [1] ABA inhibits gibberellin (synthesis / activity);
- [1] any further valid suggestion of ABA action ; e.g. DELLA not broken down / promotes dormancy / stops gene expression
- [1] auxin binds to receptor on the cell surface membrane ;
- [1] stimulates proton pumps ;
- [1] protons move from, cytoplasm / cell, to cell wall ;
- [1] pH of cell wall decreases ;
- [1] expansins activated;
- [1] loosens linkage between cellulose microfibrils ;
- [1] by breaking hydrogen bonds ; Ignore weakening bonds
- [1] K+ channels open ;
- [1] K+ diffuse into, cytoplasm / cell;
- [1] water potential (of cytoplasm / cell) decreases ;
- [1] water enters by osmosis ;
- [1] increase in turgor pressure / volume of cell increases ;
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