Before you proceed, read carefully through the whole of Question 1 and Question 2. Plan the use of the two hours to make sure that you finish the whole of Question 1 and Question 2. If you have enough time, think about how you can improve the confidence in your results, for example by recording one or more additional measurements. You will gain marks for recording your results according to the instructions. Plants transport sucrose through vascular bundles in stems and roots. You are required to investigate the movement of sucrose solution. The apparatus will be set up as shown in Fig. 1.1, using a large test-tube and a 5cm³ syringe. [Figure 1.1] You are provided with the materials shown in Table 1.1. [Table 1.1]

1. (a)(i)Complete Table 1.2. [Table 1.2 with columns 'contents of large test-tube' and 'observations', and rows 'without distilled water' and 'with distilled water'][1]
2. (a)(ii)State a tissue in a plant vascular bundle in which the same type of movement occurs as that observed in (a)(i) when the large test-tube contains distilled water. Give a reason for your answer. tissue reason[1]
3. (b)(i)Describe how you will use the apparatus provided to find the volume of distilled water, W, needed to fill the large test-tube to the mark, when the syringe is in place.[2]
4. (b)(ii)Find the volume of distilled water, W, needed to fill the large test-tube to the mark, using the method you described in (b)(i). volume[1]
5. (b)(iii)A student suggested the hypothesis that: the rate of movement of the sucrose solution from the syringe into the water in the large test-tube will decrease with time. If the student's hypothesis is correct, describe the expected trend in the time taken for the first colour change to occur when each solution collected in the large test-tubes S2, S4, S6 and S8 is heated with Benedict's solution.[1]
6. (b)(iv)Record your results in an appropriate table.[5]
7. (b)(v)The student's hypothesis stated that: the rate of movement of the sucrose solution from the syringe into the water in the large test-tube will decrease with time. State whether your results provide evidence to support or reject this hypothesis. Explain how your results provide evidence for this decision. support or reject explanation[1]
8. (c)(i)Plot a graph of the data in Table 1.4 on the grid provided. Use a sharp pencil for drawing graphs. [Grid][4]
9. (c)(ii)Use your graph to find the rate of movement of sucrose solution at 5 minutes. Show on the graph how you determined your answer. rate of movement[2]
10. (c)(iii)The procedure investigated how the rate of movement of sucrose solution from the syringe changed with time. The procedure can be modified to investigate the effect of sucrose concentration, instead of time, on the rate of movement of sucrose solution. In the modified procedure, the sucrose solution from the syringe only needs to be collected once. The time period over which the sucrose solution is collected in the procedure needs to be standardised. Use the graph to suggest a suitable time period for collecting the sucrose solution from the syringe. Give a reason for your answer. time period reason[1]
11. (c)(iv)Think about how else you could modify this procedure to investigate the effect of using different concentrations of sucrose on the rate of movement of the sucrose solution. State the concentrations of sucrose solution you would use. Describe how the concentrations of sucrose solution would be prepared.[2]

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