Phosphatases are enzymes that catalyse the removal of phosphate from other molecules, releasing inorganic phosphate (P₁). A phosphatase can be extracted from mung bean seedlings, Vigna radiata. [Figure 1.1] A student found a published method to extract phosphatase from mung bean seedlings. • Use a pestle and mortar to grind mung bean seedlings, with a small volume of distilled water, to make a paste. • Add distilled water to the paste to make a mixture with a total volume of 50 cm³. • Filter the mixture and put the filtrate into a clean centrifuge tube. • Centrifuge the filtrate until a solid pellet is formed, as shown in [Figure 1.2]. • Pour the liquid extract containing phosphatase into a clean test-tube.