Fusicoccin is a chemical that increases the sucrose concentration in phloem at the source. In this investigation you will be provided with a sample, U, which has been taken from the phloem of a plant. You will need to: • prepare proportional dilutions of a 10% sucrose solution • carry out the test for non-reducing sugars on each concentration of sucrose solution • carry out the test for non-reducing sugars on the unknown concentration of sucrose, U • estimate the concentration of sucrose in U. You are provided with the materials shown in Table 1.1. Table 1.1 labelled contents hazard volume/cm³ S 10% sucrose solution none 50 Benedict's Benedict's solution harmful 30 W distilled water none 50 H dilute hydrochloric acid irritant 20 A 10g sodium hydrogencarbonate powder none – U solution of unknown sucrose concentration none 10 If any of the materials come into contact with your skin, wash off immediately under cold water. It is recommended that you wear suitable eye protection.